Chromosomal transformation of Escherichia coli recD strains with linearized plasmids.
نویسندگان
چکیده
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 171 5 شماره
صفحات -
تاریخ انتشار 1989